Composite

Part:BBa_K2009602:Design

Designed by: Yin Wu   Group: iGEM16_USTC   (2016-09-14)


sfGFP11 with promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 68


Design Notes

If we PCR from GFP directly, we have to mutate it many times and it is more convenient to directly synthese it.


Source

chenical synthesis and link it to BBa_K081005

References